Kamiya丨Kamiya艾美捷抗FLAG多克隆说明书
Kamiya艾美捷抗FLAG多克隆化學性質:
程序:用FLAG肽免疫家兔與KLH偶聯。多次免疫后在弗氏佐劑中收集血清使用固定在固相上的肽。
規范:
使用氨基末端分析抗體Met FLAG BAP、氨基末端FLAG-BAP和羧基末端FLAG-BAP融合蛋白和Invitrogen Posite R900-40。
宿主:兔子
格式:液體PBS中的親和純化抗體,pH 7.2含0.1%疊-氮-化鈉。
Kamiya艾美捷抗FLAG多克隆儲存和穩定性:
在+4°C下儲存。
應用和建議的稀釋液:
ELISA:在1:5000-1:20000時使用。
蛋白質印跡:使用1:5000-1:100000。
特定應用的最佳稀釋度應由研究人員確定。
僅用于體外研究。不適用于診斷或人類。
Kamiya艾美捷抗FLAG多克隆英文說明:
Procedure:
Rabbits were immunized with the FLAG peptide?conjugated to KLH. After multiple immunizations
in Freund’s adjuvant, serum was collected and?the antibodies were affinity-purified using the
peptide immobilized on a solid phase.
Specification:
Antibody was analyzed using amino-terminal?Met-FLAG-BAP, amino-terminal FLAG-BAP and?carboxy-terminal FLAG-BAP fusion proteins and?Invitrogen Positope R900-40.
Host: Rabbit
Format:
Liquid. Affinitiy-purified antibody in PBS, pH 7.2?with 0.1% sodium azide.
Storage and Stability:
Store at +4°C.
Applications and Suggested Dilutions:
ELISA: Use at 1:5,000-1:20,000.
Western blot: Use at 1:5,000-1:10,000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
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